Buffered Charcoal Yeast Extract Agar Medium (BCYE Agar Medium) M813I

Jual Buffered Charcoal Yeast Extract Agar Medium (BCYE Agar Medium)
Jual BCYE Agar Medium Bersertifikat Halal
Code: M813I-500G
Brand: HIMEDIA
Packing: 500G

Recommended for selective isolation and cultivation of Legionella species from cooling towers, water samples, `clinical and other materials. The composition and performance criteria of this medium are as per the specifications laid down in ISO 11731-2017.

Feeley et al (5) originally formulated Charcoal Yeast Extract (CYE) Agar. This medium was a modification of the existing FG Agar (6). F-G Agar had starch and tryptone as ingredients in the composition. Feely et al (5, 6) replaced these two with charcoal and yeast extract respectively, and reported better recovery of Legionella pneumophilla. Later Paseulle (11) reported that supplementation of the Charcoal Yeast Agar with ACES buffer improved the performance of the medium. Edelstein (4) further modified the medium by adding alpha-ketoglutarate. This addition helped in improving the sensitivity of the medium. The formulation of Buffered Charcoal Yeast Extract Agar Base is as per specification laid in ISO 11731-2 (13). Legionella species are non-spore forming, narrow, gram-negative rods. Legionella causes pneumonia (Legionnaires disease) (3) or a milk, febrile disease (Pontiac fever). They do not oxidize or ferment carbohydrates in conventional media or grow on sheep blood agar. Growth is much better and more rapid on Buffered Charcoal Yeast Extract Agar (6, 9)

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